In case of indirect approach in regulatory analysis of genes
associated with psoriasis; the genes are paired with the associated
miRNAs and Transcription Factors (Table 1).
Further the genes, miRNAs and proteins in indirect approach
were subjected to network analysis and the further details about the
statistical methods were given below
Network construction & analysis (Cytohubba)
Genes and their regulators were subjected to the analysis by
various statistical methods (Degree, Edge Percolated Component,
Maximum Neighborhood Component, Density of Maximum
Neighborhood Component, and Maximal Clique Centrality with
six centralities Bottleneck, EcCentricity, Closeness, Radiality,
Betweenness and Stress) to identify their connectivity.
In case of MCC analysis in direct approach; VDR, MTHFR, GSTP1,
ABCC1, TYMS and SLC19A1 were ranked from 1 to 6 respectively.
Then; CYP1A2 and HLA-B were ranked 7. Finally; MAX and MYC were
ranked 9. VDR polymorphism is associated with Psoriasis (Figure 1).
In case of DMNC analysis in direct approach; MTHFR, AHR, ARNT,
CUX1, E2F1, EP300, hsa-miR-103, hsa-miR-107, hsa-miR-125a-3p
and hsa-miR-138 were ranked 1. MTHFR polymorphism is a possible
factor for reducing the clinical severity of psoriasis (Figure 2).
In case of MNC analysis in direct approach; MTHFR, AHR, ARNT,
CUX1, E2F1, EP300, hsa-miR-103, hsa-miR-107, hsa-miR-125a-3p
and hsa-miR-138 were ranked 1. MTHFR polymorphism is a possible
factor for reducing the clinical severity of psoriasis (Figure 3).
In case of degree analysis in direct approach; VDR, MTHFR,
GSTP1, ABCC1, TYMS and SLC19A1 were ranked from 1 to 6
respectively. Then; CYP1A2 and HLA-B were ranked 7. Finally; MAX
and MYC were ranked 9. VDR polymorphism is associated with
Psoriasis (Figure 4).
In case of EPC analysis in direct approach; MTHFR, VDR, SLC19A1,
GSTP1, MAX, MYC, ABCC1, hsa-miR-24, TYMS and USF1 were ranked
from 1 to 10 respectively. MTHFR polymorphism is a possible factor
for reducing the clinical severity of psoriasis (Figure 5).
In case of Bottleneck Analysis in direct approach; VDR, hsamiR-24 and MTHFR were ranked from 1 to 3. Then, MYC and GSTP1
were ranked 4. Further; ABCC1, TYMS and STAT1 were ranked from
6 to 8. Finally, YY1and HLA-B were ranked 9. VDR polymorphism is
associated with Psoriasis (Figure 6).
In case of Eccentricity Analysis in direct approach; JUN, MAX,
MYC, CREB1 and hsa-miR-24 were ranked
Then; MTHFR, SLC19A1, TYMS, VDR and GSTP1 were ranked 6.
JUN is associated with psoriasis because it’s involved in the epidermal
signaling (Figure 7).
In case of Closeness Analysis in direct approach; VDR, MTHFR,
MAX, hsa-miR-24 and JUN were ranked from 1 to 5 respectively. Then,
MYC and GSTP1 were ranked 6. ABCC1, TYMS and SLC19A1 were
ranked from 8 to 10 respectively. VDR polymorphism is associated
with Psoriasis (Figure 8).
In case of Radiality Analysis in direct approach; MAX, hsa-miR-24,
VDR, MTHFR, JUN, MYC, GSTP1, TYMS, SLC19A1 and ABCC1 were
ranked from 1 to 10 respectively (Figure 9).
In case of Betweenness Analysis in direct approach; VDR, MTHFR,
GSTP1, MAX, hsa-miR-24, ABCC1, TYMS, MYC, HLA-B and JUN were
ranked from 1 to 10 respectively. VDR polymorphism is associated
with Psoriasis (Figure 10).
In case of Stress Analysis in direct approach; MTHFR, VDR,
GSTP1, MAX, CYP1A2, ABCC1, hsa-miR- 24, HLA-B, MYC and SLC19A1
were ranked from 1 to 10. MTHFR polymorphism is a possible factor
for reducing the clinical severity of psoriasis (Figure 11).
In case of Clustering coefficient Analysis in direct approach;
MTHFR, AHR, ARNT, CUX1, E2F1, EP300 hsa-miR-103, hsa-miR-107,
hsa-miR-125a-3p and hsa-miR-138 were ranked from 1 to 10. MTHFR
polymorphism is a possible factor for reducing the clinical severity of
psoriasis (Figure 12) (Table 2).
